Regulatory

Part:BBa_K1051303:Design

Designed by: Kequan Lin   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)


clb6 promoter (during G1 phase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : 

upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT

downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG


Source

TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA

References